DETEKSI VIRUS HEPATITIS E (HEV) DAN HANTAVIRUS PADA INANG RESERVOIR (TIKUS) DI KABUPATEN KLATEN DAN KENDAL, PROVINSI JAWA TENGAH

Vektora : Jurnal Vektor dan Reservoir Penyakit Pub Date : 2019-09-25 DOI:10.22435/vk.v11i2.2104

Arief Mulyono, Tika Fiona Sari, Ristiyanto Ristiyanto, B. Yuliadi, Edi Royandi, A. Pratiwi

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Abstract

Rats are animal reservoirs and harbours of several zoonotic pathogens diseases in humans. At least, there are 68 viruses of zoonotic agents that can be transmitted by rats. Two common types of viruses attackinghumans are Hepatitis E virus (HEV) and Hantavirus. Early detection of those viruses is fundamentally required in order to prevent disease transmissions to humans. The aim of the study was to detect and count the percentage of rats infected by HEV and Hantavirus in Kendal and Klaten Districts, Central Java Province. The research design used in this study was descriptive design with cross-sectional approach. The target population was rats distributed in Klaten and Kendal Districts. In addition, the research subject was trapped rats. Detection of Hantavirus was carried out using ELISA method and detection of HEV was conducted using nested reverse transcription PCR (nested RT-PCR). A total of 73 rats was successfully captured consisting of 2 genus and 4 species e.g. Rattus novergicus, R. tanezumi, R. tiomanicus and Bandicota indica. The trapped rats infected by HEV were 3.7% and 41.3% for Klaten and Kendal, respectively. However, the seropositive of Hantavirus was only found in Kendal District (20.5%). Rat control is necessary to prevent transmission of HEV and Hantavirus.

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大鼠是人类几种人畜共患病原体疾病的动物宿主和宿主。至少有68种人畜共患病毒可以通过老鼠传播。攻击人类的两种常见病毒是戊型肝炎病毒(HEV)和汉坦病毒。为了防止疾病传播给人类，早期发现这些病毒是必不可少的。本研究的目的是检测和计数中爪哇省肯德尔区和克拉丹区感染戊型肝炎病毒和汉坦病毒的大鼠百分比。本研究采用横断面方法的描述性设计。目标人群为分布在克拉丹区和肯德尔区的大鼠。另外，研究对象为诱捕大鼠。采用ELISA法检测汉坦病毒，采用巢式反转录PCR (nested RT-PCR)检测HEV。共捕获褐家鼠、黄胸鼠、tiomanicus、印度斑腹鼠2属4种73只。Klaten和Kendal的捕获鼠感染HEV率分别为3.7%和41.3%。汉坦病毒血清阳性仅在肯德尔区(20.5%)出现。控制大鼠是防止戊肝病毒和汉坦病毒传播的必要措施。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Vektora : Jurnal Vektor dan Reservoir Penyakit

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