COMPARATIVE ORAL SUSCEPTIBILITY OF DENGUE VECTOR MOSQUITOES FROM JAPAN AND SOUTHEAST ASIA TO TWO DENGUE-1 VIRUS STRAINS

H. Ishak, I. Matsuse, K. Kamimura, T. Takegami, H. Funada
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Abstract

Five geographic strains of Aedes albopictus from Japan and Southeast Asia and three strains of Aedes aegypti from Indonesia and Pakistan were compared for their susceptibility to oral infection with the human virus, dengue-1 Mochizuki strain (isolated in 1943, Japan) and A88 strain (isolated in 1988, Indonesia). Female mosquitoes, aged 3-4 days, were infected with a virus-erythrocyte-sucrose suspension. After 14 days of incubation at 25-30°C, viral infection in mesenteron (midgut) or in head-thorax (salivary gland) of each individual mosquito was identified by the reverse transcriptase-polymerase chain reaction (RT-PCR) amplification of dengue-1 virus envelope gene. Although the results of susceptibility varied in some extent in different strains of mosquitoes and viral strains, the oral susceptibility to both dengue-1 virus strains was not significantly different among dengue vector mosquitoes from Japan and Southeast Asia.
日本和东南亚登革热媒介蚊对两种1型登革热病毒株的口腔易感性比较
比较了来自日本和东南亚的5株白纹伊蚊和来自印度尼西亚和巴基斯坦的3株埃及伊蚊对人类口腔感染病毒的易感性,分别是日本1943年分离的denu -1 Mochizuki株和印度尼西亚1988年分离的A88株。用病毒-红细胞-蔗糖悬浮液感染3-4日龄雌蚊。在25-30℃条件下孵育14 d后,采用逆转录聚合酶链反应(RT-PCR)扩增法检测登革-1型病毒包膜基因在每只蚊子肠系膜(中肠)或头胸(唾液腺)内的病毒感染情况。虽然不同蚊种和病毒株的敏感性结果存在一定差异,但日本和东南亚登革热媒介蚊对两种登革热-1病毒株的口腔敏感性无显著差异。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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