Comparative physiological and serotoninergic properties of pulvinar neurons in the monkey, cat and ferret

Abstract

The basic electrophysiological properties and responses to serotonin of thalamocortical (TC) neurons in the ferret, cat, and monkey pulvinar were compared. Morphologically, thalamocortical neurons in these three species were similar, except for the presence of beaded dendrites in many monkey neurons. In all three species, the neurons exhibited two distinct firing modes: single spike activity and low threshold Ca²⁺-spike mediated bursting. However, in monkeys, the low threshold Ca²⁺ spikes were followed by a prominent 50–100 ms afterhyperpolarization that could result in the generation of an additional rebound Ca²⁺ spike. The application of 5-HT to thalamocortical neurons in cat and monkey pulvinar resulted in a depolarization and an increase in membrane conductance through an enhancement of the hyperpolarization-activated cation current, I_h, apparently through the activation of 5-HT₇ receptors. In contrast, the application of serotonin to ferret pulvinar neurons resulted in a prominent hyperpolarization, owing to an increase in membrane potassium conductance. In monkey and ferret, application of serotonin could result in barrages of IPSPs in thalamocortical neurons. These results indicate that there are significant species-dependent differences in both the electrophysiological and pharmacological properties of pulvinar thalamocortical neurons.