MULTIPLIKASI Aquilaria malaccensisDENGAN NAA DAN RAGI PADA KULTUR IN VITRO

Samanhudi Samanhudi, Amalia Tetrani Sakya, E. Purwanto, Indah Retnosari
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引用次数: 1

Abstract

Multiplication of Aquilaria malaccensis with naa and yeast growth regulators on in vitro culture. This study aims to obtain the best concentration of NAA and yeast extract for multiplication of agarwood on in vitro culture. This research was conducted from January to October 2020 at the Laboratory of Plant Physiology and Biotechnology, Faculty of Agriculture, Universitas SebelasMaret, Surakarta. The experimental design used was a factorial completely randomized design (CRD) with twofactors, namely NAA (0 ppm; 0.1 ppm; 0.2 ppm; and 0.3 ppm) and yeast extract (0 mg/l, 700 mg/l, 800 mg/l, and 900 mg/l).(+)The results showed that the combination of 0 ppm NAA and 900 mg/lyeast increasedthe number of shoots of A.malaccensis explants with the highest average number of 3.67 shoots. A single NAA concentration of 0 ppm was able to increase the number of leaves of explants of A. malaccensis with the highest average leaf rate of 24.5 leaves. A single yeast concentration of 0 mg/lwas able to increase the number of leaves of A. malaccensis explants with an average of 22 leaves.
在体外培养中加入马拉基菌和酵母
naa和酵母生长调节剂对黑木香体外培养增殖的影响。本研究旨在获得沉香体外培养增殖的最佳NAA和酵母浸出物浓度。该研究于2020年1月至10月在泗水SebelasMaret大学农业学院植物生理学和生物技术实验室进行。试验设计采用因子完全随机设计(CRD),有两个因素,即NAA (0 ppm;0.1 ppm;0.2 ppm;(+)结果表明,0 ppm NAA和900 mg/l酵母提取物的组合处理均能增加马拉卡松外植体的芽数,平均芽数最高,为3.67个。单次NAA浓度为0 ppm时,马六甲外植体叶片数增加,平均叶片率最高,达24.5片。单个酵母浓度为0 mg/l时,可使马六甲外植体叶片数量平均增加22片。
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