Stromal sodium binding after glycosaminoglycan digestion.

Investigative ophthalmology Pub Date : 1976-06-01
K Green, S Downs, K Bowman
{"title":"Stromal sodium binding after glycosaminoglycan digestion.","authors":"K Green,&nbsp;S Downs,&nbsp;K Bowman","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>The glycosaminoglycans of isolated rabbit corneal stroma, clamped between two lucite plates at near normal hydration, were digested with testicular hyaluronidase in saline solution. After equilibration with 0.9 per cent saline solution alone the sodium and chloride content of the stroma was determined. Chloride was in equilibrium with both normal and hyaluronidase-treated stroma, allowing use of the Donnan calculation for excess or bound sodium to be made. Normal stromas contained 200 mEq. bound sodium per kilogram of dry weight calculated from the Donnan calculation; hyaluronidase-treated stromas contained 110 mEq. bound sodium per kilogram of dry weight. The data show that about half of the bound sodium in the corneal stroma is on nonsaccharide binding sites. Quantitative verification of the loss of glycosaminoglycans was performed.</p>","PeriodicalId":14844,"journal":{"name":"Investigative ophthalmology","volume":"15 6","pages":"484-6"},"PeriodicalIF":0.0000,"publicationDate":"1976-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Investigative ophthalmology","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

Abstract

The glycosaminoglycans of isolated rabbit corneal stroma, clamped between two lucite plates at near normal hydration, were digested with testicular hyaluronidase in saline solution. After equilibration with 0.9 per cent saline solution alone the sodium and chloride content of the stroma was determined. Chloride was in equilibrium with both normal and hyaluronidase-treated stroma, allowing use of the Donnan calculation for excess or bound sodium to be made. Normal stromas contained 200 mEq. bound sodium per kilogram of dry weight calculated from the Donnan calculation; hyaluronidase-treated stromas contained 110 mEq. bound sodium per kilogram of dry weight. The data show that about half of the bound sodium in the corneal stroma is on nonsaccharide binding sites. Quantitative verification of the loss of glycosaminoglycans was performed.

糖胺聚糖消化后基质钠结合。
将离体兔角膜基质的糖胺聚糖夹在两个接近正常水合状态的玻璃石板之间,用睾丸透明质酸酶在盐水溶液中消化。用0.9%的盐水溶液平衡后,测定基质的钠和氯化物含量。氯化物与正常和透明质酸酶处理的基质均处于平衡状态,允许使用Donnan计算过量或结合的钠。正常基质含200meq。根据Donnan计算得出的每公斤干重的束缚钠;透明质酸酶处理的基质含有110 mEq。每公斤干重所含的束缚钠。数据表明,角膜基质中约有一半的结合钠位于非糖结合位点。定量验证了糖胺聚糖的损失。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信