D. Aceti, Paul G. Blommel, Y. Endo, B. Fox, R. Frederick, A. Hegeman, W. Jeon, Todd Kimball, Jason M. Lee, Craig S. Newman, F. Peterson, T. Sawasaki, K. Seder, M. Sussman, E. Ulrich, R. Wrobel, S. Thao, D. Vinarov, B. Volkman, Qin Zhao
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引用次数: 11
Abstract
Introduction
Overview of Structural Genomics Initiatives World-wide
Use of Bioinformatics as a Predictive Tool
High-throughput Cloning Strategies Applied to Arabidopsis
cDNA Sources
DNA Chip Technologies as a Predictive Tool
Genes Lacking Introns
Restriction Digestion and Ligation
Topoisomerase Cloning
Transformation
Sequence Verification
Escherichia coli Expression Systems
T7 Promoter Systems
Arabinose Operon Promoter System
Wheat Germ Cell-free Translation Systems
Characterization of Expression, Solubility, and Purity
Electrophoresis and Dye Binding
ELISA-based Detection
S-Tag-Based Detection
Protein Tags and Multi-step Purification Procedures
Role of Protease Cleavage in Target Preparation
Protein Characterization by Mass Spectrometry
Integration with Broader Biological Sciences Community
Acknowledgments
Keywords:
affinity tag;
Arabidopsis thaliana;
bioinformatics;
catabolite repression;
codon bias;
codon usage;
Escherichia coli;
expression vectors;
factor Xa;
fusion protein;
gene expression;
heterologous expression;
high-density oligonucleotide arrays;
Laboratory Information Management Systems;
MALDI-TOF;
mass spectrometry;
peptide mapping;
PreScission;
protease cleavage;
protein sequence analysis;
solubility tag;
soluble protein;
structure and functional biology;
TEV;
T7 bacteriophage promoter;
T7 RNA polymerase;
target selection;
thrombin;
transcriptome
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