PATHOLOGICAL AND MOLECULAR EVALUATION OF CANINE PARVOVIRUS IN SOUTH OF IRAQ

Abstract

This study is the first in southern Iraq, which shows the diagnosis of Parvovirus disease in young puppies, where the samples were collected from small dogs, and this was done from April 2018 to April 2019 it was conducted on 50 dogs with different breeds ranging between (2-18) months aged of both sexes. fecal samples were collected from infected dogs from veterinary clinics in (Basrah, Dhi Qar, Maysan and AL-Muthanna provinces) .After the autopsy of the dead carcass which died soon during the period (24-72) hours, bleeding in the intestines and congestion was observed as well as the water content and adhesions, hypertrophy of the heart and necrosis in areas of pale color of different size focci are surrounded by hyperemic zone. The microscopical result of enteric CPV lesion showed sever infiltration of inflammatory cells in the mucosal layer of intestine ,hypertrophy of goblet cells in the villi and necrotizing area in the tip of villi as well to hyperplasia of goblet cells also edematous fluid in mucosal layer. cardiac CPV lesion showed infiltration of inflammatory cells in myocardial cell fibers ,also there was an area of vacculation of some myocardial cells in addition to edematous fluid in the myocardial interstitiaum .significant increases of biochemical cardiac markers(cTi-1,AST,LDH and CPK) showed cardiac troponin I (cTnI), aspartate transaminase AST, creatinine and the Parvovirus contains 400 bp of matrimonial bases after being diagnosed using the polymerase chain reaction PCR and this technical method widely applied to provide rapid diagnosis The current study conducts that the CPV-2 is endemic in south of Iraq ,the disease shows very important clinical and biochemical feature that lead to morbidity