Deoxyribonucleic Acid Base Composition and Nucleotide Distribution of Pedomicrobium spp.

Zentralblatt für Bakteriologie Mikrobiologie und Hygiene: I. Abt. Originale C: Allgemeine, angewandte und ?kologische Mikrobiologie Pub Date : 1981-12-01 DOI:10.1016/S0721-9571(81)80026-9

Rainer Gebers , Manley Mandel , Peter Hirsch

引用次数: 13

Abstract

The DNAs of seven bacterial strains belonging to Pedomicrobium ferrugineum and P. manganicum were investigated by buoyant density centrifugation and thermal denaturation experiments. The DNA base composition was calculated to range from 66.3 to 67.3% GC in CsCl gradients and from 64.8 to 65.7% GC in thermal transition profiles. The correlation of both methods was T_mo.issc = 428.5 (ϱ — 1.533) under our experimental conditions. Analysis of the DNA nucleotide distributions revealed a homogeneous group characterized by Gaussian distribution with a mean width of 9.19 ± 0.14% GC (uncorrected) and an average degree of asymmetry of 1.16 ± 0.04.

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土壤微生物的脱氧核糖核酸碱基组成及核苷酸分布。

采用浮力密度离心和热变性实验研究了7株铁磷微生物菌(Pedomicrobium ferrugineum)和锰磷微生物菌(P. manganium)的dna。DNA碱基组成在CsCl梯度中为66.3 ~ 67.3%，在热转变剖面中为64.8 ~ 65.7%。两种方法的相关性为Tmo。在我们的实验条件下Issc = 428.5 (ϱ - 1.533)。对DNA核苷酸分布进行分析，发现同质组具有高斯分布特征，平均宽度为9.19±0.14% GC(未校正)，平均不对称度为1.16±0.04。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Zentralblatt für Bakteriologie Mikrobiologie und Hygiene: I. Abt. Originale C: Allgemeine, angewandte und ?kologische Mikrobiologie

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