Does OTC inhibited glutamate uptake by GLAST and GLT-1?

Abstract

Ochratoxin A, a naturally occurring toxin that is produced by fungi and proven to be able to cause nephrotoxic, teratogenic, and carcinogenic problems via oxidative DNA damage. Ochratoxin C is proven to be able to convert into Ochratoxin A in mice, which also inhibits the absorption of glutamate by astrocytes through a decrease in cell surface expression of the excitatory amino-acid transporters GLAST and GLT-1. This paper is to explore the influence of different amounts of Ochratoxin C treating different types of mice, while measuring glutamate or glutamine uptake in neurons. Investigate Ochratoxin C affinity column pulldown to check for interaction between Ochratoxin C and GLAST or GLT1. In this paper, study will show whether the Ochratoxin C shows the same reaction of Ochratoxin A, showing that Ochratoxin C should get more attention on the future clinical trial just like Ochratoxin A. Further studies will focus on the regulation of Ochratoxin C to see if Ochratoxin A could be regulated as well when they could be converted.