Effect of zinc ions on the oxygen affinity of hemoglobin and the level of 2,3-DPG in normal human red blood cells in vitro.

Zentralblatt fur Bakteriologie, Parasitenkunde, Infektionskrankheiten und Hygiene. Erste Abteilung Originale. Reihe B: Hygiene, Betriebshygiene, praventive Medizin Pub Date : 1979-01-01

A I Kabat, J Niedworok, J Kedziora, J Błaszczyk, G Bartosz

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Abstract

This paper presents the results of experiments on the in vitro effect of zinc ions on the affinity of hemoglobin for oxygen and on the 2,3-DPG content of the red blood cells of healthy humans. Blood was incubated in 1.5 X 10(-3) M ZnSO4 solution for 4 h and then the oxygen affinity of hemoglobin and the hematocrit number were determined. An original method of determination the oxygen dissociation curve of HbO2 was employed enabling an exact calculation of the P50 value of the hemolysates by the method of least squares. Analysis of statistically verified results reveals that zinc ions increase the oxygen affinity of hemoglobin bringing about a leftward shift of the HbO2 dissociation curve (by 3.85 mm Hg for P50) the level of 2,3-DPG remaining constant in the red cells incubated with ZnSO4. The mechanisms of this phenomenon and its possible clinical implications are discussed.

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锌离子对体外正常人红细胞血红蛋白氧亲和力及2,3- dpg水平的影响

本文介绍了锌离子对体外健康人血红蛋白对氧亲和力及红细胞2,3- dpg含量影响的实验结果。血液在1.5 × 10(-3) M ZnSO4溶液中孵育4 h，测定血红蛋白的氧亲和力和红细胞压积数。采用了一种测定HbO2氧解离曲线的原始方法，可以用最小二乘法精确计算溶血物的P50值。经统计验证的结果分析表明，锌离子增加血红蛋白的氧亲和力，使HbO2解离曲线向左移动(P50时移动3.85 mm Hg)，而用ZnSO4孵育的红细胞中2,3- dpg水平保持不变。本文讨论了这种现象的机制及其可能的临床意义。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Zentralblatt fur Bakteriologie, Parasitenkunde, Infektionskrankheiten und Hygiene. Erste Abteilung Originale. Reihe B: Hygiene, Betriebshygiene, praventive Medizin

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