Observation of the permeation on the subcutaneous during the administration of drug and development of a needle

Abstract

In recent years, the number of diabetics in Japan is increasing every year. During the treatment, patients with diabetes are doing injections several times a day for insulin administration. Compared with the other injection method into the subcutaneous tissue, the hypodermic injection which the effect of a medicine maintains more is used in that case. However, there are few study cases about the percolation process of the medicine at the time of a hypodermic injection, design of the needle in consideration of the efficiency of drug penetration as well as durability despite being sought, the design of the hypodermic needle for medication which a medicine is made to permeate efficiently is not yet made. Therefore, confirmation of subcutaneous infiltration process has become an issue. Then, in order to check change of the osmosis tendency accompanying a check of time progress for the percolation process at the time of medication, the fluorescence reagent was used and the osmosis observation experiment was conducted. With a syringe of 27G on the back of surviving nude mouse, in order to confirm the presence or absence of a difference to the fluorescence reaction, it was observed fluorescence reaction of the elapsed time intervals using a IVIS Lumina II administered two fluorescent reagents. A result of the osmosis observation experiment, we confirmed that FITC was suitable for observation.