Biodegradable nanoparticles of alginate and chitosan as non-viral DNA oral delivery system

Lídia M D Gonçalves, A. Cadete, L. Figueiredo, C. Calado, A. Almeida
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引用次数: 4

Abstract

The delivery of nucleic acids via the oral route involves overcoming barriers such as degradation of nucleic acids by low pH in the stomach, enzymatic degradation by DNases in the gut, crossing the physical barrier imposed by the mucus layer, cellular uptake, intracellular trafficking and nuclear uptake. As an oral drug carrier system chitosan nanoparticles are ideal, being mucoadhesive, interacting with the anionic sialic acid residues in mucin. In this study, plasmid DNA expressing a “humanized” secreted Gaussia Luciferase as reporter gene was encapsulated in alginate and chitosan nanoparticles, via a mild ionotropic gelation procedure with sodium tripolyphosphate as a counterion. The nanoparticle system here developed shows effective transfection of different human gastric epithelial cell lines with distinct cell differention. That was confirmed by the expression of luciferase in the different tested conditions, particularly the amount of encapsulated pGLuc.
可生物降解的海藻酸盐和壳聚糖纳米颗粒作为非病毒DNA口服递送系统
通过口服途径递送核酸需要克服一些障碍,如胃内低pH值对核酸的降解、肠道内dna酶的酶降解、跨越黏液层施加的物理屏障、细胞摄取、细胞内运输和核摄取。壳聚糖纳米颗粒是一种理想的口服药物载体系统,具有粘接性,可与粘蛋白中的阴离子唾液酸残基相互作用。在这项研究中,表达“人源化”分泌的Gaussia荧光素酶作为报告基因的质粒DNA被包裹在海藻酸盐和壳聚糖纳米颗粒中,通过温和的离子化凝胶化过程,以三聚磷酸钠作为反离子。该纳米颗粒系统转染不同的人胃上皮细胞系,细胞分化明显。荧光素酶在不同测试条件下的表达,特别是包封pGLuc的量,证实了这一点。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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