DETECTION AND LABORATORY FINDINGS DUE TO EHRLICHIA CANIS IN DOGS FROM THE SOUTH AREA OF SÃO PAULO – SP, BRAZIL

Stephanny Eringis de Queiroz, Daniel Moura Aguiar, Edilson Isídio da Silva Junior, D. Reggiani, Tânia Regina Vieira de Carvalho, Rafael Garabet Agopian, Kleber da Cunha Peixoto Junior, A. Cortez, J. Moraes Filho
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Abstract

OBJECTIVE: To evaluate hematological changes in dogs diagnosed with Ehrlichia canis. METHODS: 54 dogs treated at the Veterinary Hospital of the Santo Amaro University were analyzed. During the consultation, the animals' real temperature was measured; and venous blood samples were collected for hemogram; and performed serological and molecular tests, through Immunofluorescence and PCR in real time, respectively. RESULTS: Among the 54 animals suspected of having E. canis, 32 (59.3%) were diagnosed as positive. Of these, 23 (42.6%) were positive only in the serological test, while 9 (16.7%) had positive results in the serological and molecular tests. Thrombocytopenia was the most important alteration found in this study and was the only parameter found in the blood count that showed a statistical difference. CONCLUSION: It was concluded that thrombocytopenia was the main finding in infected animals and that the combination of molecular and serological diagnoses may increase the chances of detection of infection or exposure to the agent.
巴西sÃo paulo - sp南部地区犬中犬埃利希体的检测和实验室结果
目的:评价犬埃利希体血液学变化。方法:对圣阿马洛大学兽医医院收治的54只犬进行分析。在咨询过程中,测量了动物的真实体温;采集静脉血做血象图;并分别通过免疫荧光和实时PCR进行血清学和分子检测。结果:在54只疑似犬e型感染的动物中,32只(59.3%)被诊断为阳性。其中,仅血清学检测阳性23例(42.6%),血清学和分子检测均阳性9例(16.7%)。血小板减少是本研究中发现的最重要的改变,也是在血细胞计数中发现的唯一有统计学差异的参数。结论:血小板减少是感染动物的主要表现,分子诊断和血清学诊断相结合可能增加感染或接触病原体的机会。
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