Uji Aktivitas Antimikroba dan Antioksidan dari Ekstrak Etanol Daun Keji Beling (Strobilanthes crispus BI)

Talenta Conference Series: Science and Technology (ST) Pub Date : 2019-06-30 DOI:10.32734/ST.V2I2.511

R. Bulan, Firmanta Sebayang, Emma Zaidar Nasution, Putri Wulandari

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Abstract

Uji aktivitas antimikroba dari ekstrak etanol daun keji beling (Strobilanthes crispus BI) telah dilakukan terhadap mikroba Candida albicans, Microsporum gypseum, Bacillus cereus, dan Shigella dysentriae dengan metode difusi cakram. Uji aktivitas antioksidan ekstrak etanol daun keji beling dilakukan dengan metode DPPH. Daun keji beling yang telah halus, diekstraksi dengan metode maserasi menggunakan pelarut etanol 96% selama 2 hari, dan dipekatkan dengan rotary vacum evaporator. Uji aktivitas antimikroba dilakukan dengan variasi konsentrasi 100; 200; 300; 400; 500 mg/ml. Hasil skrining fitokimia ekstrak etanol daun keji beling menunjukkan hasil positif terhadap senyawa golongan alkaloid dan fenolik. Hasil penelitian menunjukkan ekstrak etanol daun keji beling efektif terhadap mikroba Candida albicans dan tidak efektif terhadap mikroba Microsporum gypseum, Bacillus cereus, dan Shigella dysentriae. Ekstrak etanol daun kejibeling mampu menghambat pertumbuhan Candida albicans dengan diameter zona bening untuk konsentrasi 100; 200; 300; 400; 500 mg/ml masing-masing adalah 11,53; 13,96; 15,46; 16,80; dan 18,20 mm. Uji aktivitas antioksidan ekstrak etanol daun keji beling dengan metode DPPH menunjukkan nilai IC50 sebesar 155,2 ppm. The antimicrobial and antioxidant activity evaluation of leaves ethanol extract of KejiBeling (Strobilanthescrispus BI) has been carried out. The leaves of KejiBeling was grounded into powder and extracted using ethanol 96% (v/v) for 2x24 h and concentrated with rotary vacum evaporator. The antimicrobial activity was performed against several microbes, i.e. Candida albicans, Microsporumgypseum, Bacillus cereus, andShigella dysentriaeusing disk diffusion method. The antimicrobial activity was performed using several concentrations of leaves extract from 100-500mg/mL.The phytochemical screening showed the presence of alkaloid and phenolic group intheleaves ethanol extract of KejiBeling. The leaves ethanol extract of KejiBeling was antimicrobial active against Candida albicans with inhibition zone diameter of 11,53; 13,96; 15,46; 16,80; and 18,20 mm for the concentration of 100; 200; 300; 400; 500 mg/ml, respectively. The antioxidant activity of leaves ethanol extract showed IC50 value of 155,2 ppm

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测试臭名昭著的白叶乙醇提取物中的抗菌素和抗氧化活性(草莓毒BI)

用聚合体扩散法对鳞茎菌、微孢子菌、芽孢杆菌、芽孢菌脑浆和磷酸二乙醇的抗菌活性进行了测试。臭名昭著的叶乙醇提取反氧化活性试验采用DPPH方法。用乙醇96%的溶剂进行了2天的切割，并与真空蒸发器转轮结合。抗菌素活性测试采用100浓度变化进行;200;300;400;500 mg / ml。从黑叶乙醇提取物中提取的植物化学物质对生物碱和苯类化合物表现出积极的结果。研究表明，臭名远端的乙醇提取物对鳞茎菌有效，对微生物gypseum、杆菌脑浆和Shigella dysentriae无效。心化叶乙醇提取物能够抑制白斑菌的生长，其直径为100浓度;200;300;400;每人500毫克/ml为11.53;13.96;15.46;16.80;18。20毫米。用DPPH方法去除乙醇提取物的活性测试显示IC50值为155.2 ppm。对叶的诽谤调查已经被考虑清楚了。污染的树叶被用来使用2x24 h的乙醇(v/v)，并集中使用真空蒸发扶轮器。抗菌素活性是对several microbial activity, i.e。antimicrobial activity使用了来自100.500mg /mL的several contration表现出来。植物化学的剧目展示了在叶乙醇和酚群内的存在。环保的乙醇extract是对堪培拉山脉的抗菌素活性，影响范围为11.53;13.96;15.46;16.80;1820毫米的集中力200;300;400;500毫克/ml，厌恶。叶乙醇渗出的反氧活性显示IC50值155.2 ppm

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Talenta Conference Series: Science and Technology (ST)

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