Rapid Propagation from Hypocotyl and Shoot Tip Explants of Sterculia foetida Linn

Journal of Advanced Agricultural Technologies Pub Date : 1900-01-01 DOI:10.18178/joaat.6.2.118-122

T. Handayani, E. Yuniastuti, D. Pinardi

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Abstract

Sterculia foetida Linn. is a large deciduous tree belonging to Sterculiaceae. Seed of S. foetida is used for treating skin diseases and rheumatism also can be used as a source of alternative bioenergy and medicine. The main problem of S. foetida cultivation is the limited supply of seed plants. Seeds development were very easy, but to produce fruit have to wait for approximately 5 years. The objective of this research was to obtain kepuh plants through direct in vitro regeneration. Hypocotyls and shoot tips explants were excised from sterile germinated seedlings and placed on shoot induction medium containing basal salts of Woody Plant Medium (WPM) and various concentrations of plant growth regulators. The results showed that the best medium of shoots elongation were WPM + 1 mgL-1 kinetin + 5 mgL-1 GA3. Highest percentage of roots (65%) occurred on WPM with 5 mgL-1 IBA which average number of roots was 3.1. Rooted plantlets were acclimatized to field conditions by placing them in pots containing sterilized and compost medium (1:1) and subsequently transferred to filed with 80% survival. 

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小扁豆下胚轴和茎尖外植体快速繁殖

田螺;是一种大的落叶树，属于蕨科。牛蒡子用于治疗皮肤病和风湿病，也可作为替代生物能源和药物的来源。黄花苜蓿栽培的主要问题是种子植株供应有限。种子的发育很容易，但结果需要等待大约5年。本研究的目的是通过直接离体再生获得可再生植株。从无菌萌发的幼苗中切除下胚轴和茎尖外植体，置于含有木本植物培养基(Woody Plant medium, WPM)基础盐和不同浓度植物生长调节剂的诱导培养基上。结果表明，芽伸长的最佳培养基为WPM + 1 mg -1 kinetin + 5 mg -1 GA3。当IBA浓度为5 mg -1时，WPM的生根率最高(65%)，平均生根数为3.1根。将生根苗置于含有灭菌和堆肥培养基(1:1)的花盆中，使其适应田间条件，然后转移到田间，成活率为80%。

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Journal of Advanced Agricultural Technologies

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