Antiviral activity of fluorinated compounds against DNA- and RNA-containing viruses

Abstract

Viral infections occupy an important in human as one of the most common and poorly controlled infections. At present, Influenza A virus (IAV) and Epstein-Barr virus (EBV) cause different infections, thereby imposing a huge toll on both human health and the economy worldwide. Therefore, screening new effective drugs is an urgent and important problem. Our study aimed to determine cytotoxicity and antiviral effect of fluorinated compounds against the Influenza A virus and Epstein-Barr virus. The cytotoxicity was studied in two cell lines by MTT assay. The cytotoxicity of the compounds the rate was 41 – 990 μg/ml in the B95-8 cell culture, which produces EBV, and 200 – 478 μg/ml in MDCK cells. Compounds 10S-47 and 10S-49 were able to inhibit reproduction of IAV with EC 50 of 38 μg/ml and 50 μg/ml, respectively. It was shown that fluorinated compounds showed low effectiveness against the Epstein-Barr virus, and inhibited the reproduction of the virus at 20% in the concentration range of 1 – 100 μg/ml. Also, we studied the potency of compound 10S-47 to make an apoptosis induction because it exhibited a significant cytotoxic effect on the growth of transformed cells. It was established that for compound 10S-47 at 40 mg/ml, the percentage of apoptotic cells exceeded the control and was 10%. The cytotoxicity of compounds was determined by MTT-method, CC 50 were in the range 41 – 990 µg/ml. The antiviral activity of the compounds was determined by RT-PCR. It was shown that only 10S-48 and 10S-49 compounds inhibit EBV reproduction, percentage of inhibition of synthesis of viral DNA was in range from 1 to 7. This data do not allow us to assign this class of compounds to promising anti-EBV agents.