The development of six-channel quantitative real-time PCR filter sets for nucleic acid detection

Abstract

Analyzed the technical requirements of filter sets applied in 6-channel Quantitative Real-time PCR nucleic acid detection, and these filters meet the requirements were designed with double sides coating on single glass substrate, while each coating was about 200 layers. These coatings were manufactured on a plasma-assisted reactive magnetic sputtering (PARMS) machine, and the thickness error is less than 0.1%. Finally, the transmittance rates of these 6-channel real-time fluorescence PCR filters are <93%, the cut-off steepness was less than 1%, and the crosstalk was less than 1% in polychromatic fluorescence PCR.