Piperlongumine combined with vitamin C as a new adjuvant therapy against gastric cancer regulates the ROS–STAT3 pathway

The Journal of International Medical Research Pub Date : 2022-04-01 DOI:10.1177/03000605221093308

Di Chen, Xinyue Wei, Ke Yang, Xinyue Liu, Yueqiong Song, Futing Bai, Yi Jiang, Yuhang Guo, R. Jha

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引用次数: 1

Abstract

Objective To investigate the effects of piperlongumine (PL) and vitamin C (VC) on signal transducer and activator of transcription 3 (STAT3) signalling in gastric cancer cell lines. Methods In vivo tumour xenograft anticancer assays were undertaken to confirm the anticancer activity of PL. Cell viability, flow cytometry and Western blot assays were undertaken to evaluate the anticancer effects of PL, VC and combinations of PL and VC in AGS and KATO III cells. Results Both PL and VC induced apoptosis and inhibited cell proliferation in AGS and KATO III cells. These effects were dependent on reactive oxygen species (ROS). PL effectively suppressed STAT3 activation while VC caused abnormal activation of STAT3. The combination of PL and VC exhibited a stronger apoptotic effect compared with either agent alone. PL reversed the abnormal activation of STAT3 by VC, which could be a key to their synergistic effect. Conclusions PL combined with VC exhibited a stronger anticancer effect by regulating the ROS–STAT3 pathway, suggesting that this combination might be a potential adjuvant therapy for gastric cancer.

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哌隆明联合维生素C作为胃癌新的辅助疗法调控ROS-STAT3通路

目的探讨胡椒明(PL)和维生素C (VC)对胃癌细胞信号转导因子和转录激活因子3 (STAT3)信号传导的影响。方法采用活体肿瘤移植抗肿瘤实验证实其抗肿瘤活性，并采用细胞活力、流式细胞术和Western blot技术评价其与VC及与VC联合作用对AGS和KATO III细胞的抗肿瘤作用。结果PL和VC均能诱导AGS和KATO III细胞凋亡，抑制细胞增殖。这些作用依赖于活性氧(ROS)。PL有效抑制STAT3的激活，VC引起STAT3的异常激活。与单独使用任何一种药物相比，PL和VC联合使用具有更强的凋亡作用。PL逆转了VC对STAT3的异常激活，这可能是它们协同作用的关键。结论PL联合VC通过调控ROS-STAT3通路具有较强的抗癌作用，可能是一种潜在的胃癌辅助治疗方法。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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The Journal of International Medical Research

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