Validation of Statistical Quality Controls to Improve HCV Detection: An Efficient Approach for Accurate Diagnosis

Abstract

HCV is the RNA enveloped virus belonging to the family of flavivirus, causing serious hepatic problems all over the world. While screening for HCV, many of the results false negative or false positive which reach chronic stages of the infection and eventually cancer. This study was conducted at molecular laboratory of University of Agriculture Faisalabad from June 2021 to September 2021. The aim of this study was to estimate various parameters like sensitivity, specificity, accuracy, likelihood ratios for HCV-RNA PCR and ELISA. All parameters for both diagnostic tools were compared. Sensitivity for HCV-RNA PPCR was 96% but for ELISA was 57%. Specificity for ELISA was 95% whereas 98% for HCV-RNA PPCR. The PPV of the HCV RNA-PCR test was calculated as 80% and 94% for PCR. Accuracy for both results varied as well showing among their efficiency and capacity. Results showed that HCV-RNA PCR were quite accurate and more reliable than the ELISA.