Amperometric sensors as basic structures for enzymatic, enzyme-cofactor mediated assays, and drugs detection

Abstract

In this paper preliminary results related to amperometric sensors fabricated in different technologies including thick- and thin-film technology are presented. The three-electrode sensors were designed as disposable ones and for monitoring. Disposable sensors were made by the screen-printing of the electrode material on polymeric foils, the other ones were made by screen-printing on ceramic or by platinum evaporation onto silicon wafer. Potential applications of the developed amperometric sensors for enzymatic, enzyme-cofactor mediated assays and phenothiazine-based drug detection were demonstrated. It was stated that, the PPy layer deposition on Pt electrode might lead to use of this material to decrease an oxidation potential of NADH in the NAD regeneration process.