3D cultivation of mesenchymal stromal cells from adipose tissue in alginate beads

Abstract

New trends of 3D printing of biological structures are still expanding and witnessing rapid rise of new knowledge. The basis for proper and successful 3D printed structures of biological materials is to create adequate biomaterial which would serve as ink for a 3D printer. Alginate is one of the materials that can be used. Alginate is a hydrogel, which is used mainly in the field of tissue engineering for its useful properties as a biomaterial. The main aim of this study was to create a biomaterial for the purposes of 3D printing and encapsulate selected type of cells to alginate beads, along with studying cell survival and stability of the biomaterial for three weeks. In this study mesenchymal stem cells (MSCs), isolated from rabbit adipose tissue, were used.