Screening of cultivation media for ldpe biodegradation by Penicillium verrucosum CNM-FP-02

Abstract

The paper’s aim was to select the optimal mineral medium for LDPE biodegradation by the strain Penicillium verrucosum CNM-FP-02. It was selected 5 mineral salt media (MSM), which differed in salt content and N/P ratio. After 40 days of submerged cultivation, the following parameters were determined: catalase activity and pH of cultural media, biomass accumulation, rate of LDPE degradation, optical microscopy and the tensile testing of polyethylene. It was observed that catalase activity on all media, except MSM 4, was higher in the presence of LDPE. The addition of polyethylene to the growth media stimulated the fungal biomass accumulation by 19.3-93.1% (4 media out of 5 tested). The percentage of degradation of LDPE films was different, depending on the culture medium, from 0.41% to 0.92%. The most active LDPE films were degraded on medium MSM 2. Visualization of plastic strips under an optical microscope revealed the immobilization of the microorganism and the damage on the polyethylene surface. The tensile test showed increased elasticity of the plastic in the variants treated with fungal strain. In conclusion, in order to stimulate the biodegradation of LDPE by the strain P. verrucosum CNM-FP-02, the medium MSM 2 (N/P ratio 1:1) was selected.