Study on milk composition and milk protein distribution in Romanian Holstein cattle

Archiva Zootechnica Pub Date : 2020-06-01 DOI:10.2478/azibna-2020-0002

R. Pelmuș, C. Lazăr, M. Palade, M. Stancu, C. Rotar, M. Gras

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Abstract

Abstract The aim of this study was to determine milk quality indices as well as the milk protein composition in Romanian Holstein cattle raised under the conditions of experimental farm of INCDBNA-IBNA. The study was carried out on 22 milk samples. The types of different milk proteins were identified by SDS-PAGE technique. Sampling day and milk chemical composition were performed during the milking period of studied cattle. The quality indices were breed-specific for protein (3.38%) and higher for fat (4.39%). Milk proteins analysis of Romanian Holstein cattle separated by SDS-PAGE electrophoresis showed the presence of four major caseins (αs1-, αs2-, β- and k-casein) and two whey proteins (β-lactoglobulin, α-lactalbumin). The caseins accounted 77.28% of the total milk proteins, while the major proteins (β-lactoglobulin, α-lactalbumin) from the whey represented 22.72% of the total proteins. αs1-casein + αs2-casein had a higher expression (36.01%) followed by β-casein (31.45%), β-lactoglobulin (18.16%), k-casein (9.82%) and α-lactalbumin (4.56%). The most of milk samples was characterized by a medium expression level of both caseins and whey proteins

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罗马尼亚荷斯坦牛乳成分及乳蛋白分布的研究

摘要本研究旨在测定在INCDBNA-IBNA实验农场条件下饲养的罗马尼亚荷斯坦牛的乳品质指标及乳蛋白组成。这项研究是在22份牛奶样本上进行的。采用SDS-PAGE技术鉴定了不同乳蛋白的类型。研究牛在挤奶期间进行了取样日和牛奶化学成分的测定。蛋白质的品质指标为品种特异性的3.38%，脂肪的品质指标较高(4.39%)。对罗马尼亚荷斯坦牛的乳蛋白进行SDS-PAGE电泳分析，发现乳蛋白中存在4种主要酪蛋白(αs1-、αs2-、β-和k-酪蛋白)和2种乳清蛋白(β-乳球蛋白、α-乳清蛋白)。酪蛋白占总乳蛋白的77.28%，而乳清中的主要蛋白(β-乳球蛋白、α-乳蛋白)占总蛋白的22.72%。αs1-酪蛋白+ αs2-酪蛋白的表达量最高(36.01%)，其次是β-酪蛋白(31.45%)、β-乳球蛋白(18.16%)、k-酪蛋白(9.82%)和α-乳清蛋白(4.56%)。大多数牛奶样品的特征是酪蛋白和乳清蛋白的中等表达水平

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Archiva Zootechnica

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