CRP single nucleotide polymorphism (rs1800947) and nutritional status in hemodialysis patients

Abstract

Introduction: C-reactive protein (CRP) is an acute phase protein and was shown to be a predictor for all-cause and cardiovascular death in end stage renal disease patients. C-reactive protein is a member of the pentraxin fam-ily and plays a key role in the innate immune response. Several single nucleotide polymorphisms (SNP) associated with plasma CRP levels have been identified. This study aimed to evaluate the frequency of the rs1800947 polymorphism in the human CRP gene in maintenance hemodialysis (HD) patients, and to determine whether it is related to nutritional status. Material and methods: We investigated 99 patients maintained on chronic hemodialysis (HD). 109 apparently healthy volunteers served as a control group. HD subjects were followed for 12 months. Anthropometric measurements were performed, the malnutrition-inflammation scale (MIS) score was determined, and blood samples were obtained within 1 month of enroll-ment and at 3 and 12 months of follow-up. rs1800947 SNP in the CRP gene was genotyped using restriction fragment length polymorphism-polymerase chain reaction. Results: There were no significant differences in the investigated genotype frequencies between HD patients and controls. Moreover, there was no influence of genotype on anthropometric and laboratory indices of nutritional status and MIS score. Conclusions: The results of this study indicate that the rs1800947 single nucleotide polymorphism in the CRP gene does not seem to correlate with nutritional status in hemodialysis patients.