Metaproteomics approaches and techniques: A review

A. Ameen, S. Raza
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引用次数: 5

Abstract

Extensive studies have been done on metagenomics for various microbial communities. The advancements in metagenomics level led to the need of metaproteomics approaches. Metaproteomics involve the identification, function and expression of various proteins present in microbial community, it also involves the identification and expression analysis of stress related proteins. The concepts of metaproteomics come with advancement in proteomics techniques which includes 2D gel electrophoresis for the identification of proteins and peptides in a particular microbial community. Mass spectrometry which is used to separate the proteins by desorption and ionization using a gas on a liquid medium. MALDI use for protein identification and separation, connected with TOF to give better results. The metaproteomics approaches become more advanced when HPLC and LC were used for peptides and protein with computational tools to sequence the peptide and protein. It is concluded that there is a requirement of research in metaproteomics. Many scientists have done research on these approaches but there is lack of better quality and desirable results.
元蛋白质组学方法和技术综述
对各种微生物群落的宏基因组学进行了广泛的研究。元基因组学水平的进步导致了对元蛋白质组学方法的需求。元蛋白质组学涉及微生物群落中各种蛋白质的鉴定、功能和表达,也涉及与胁迫有关的蛋白质的鉴定和表达分析。元蛋白质组学的概念是随着蛋白质组学技术的进步而出现的,其中包括用于鉴定特定微生物群落中蛋白质和肽的二维凝胶电泳。质谱法是用气体在液体介质上解吸和电离来分离蛋白质的方法。MALDI用于蛋白质的鉴定和分离,与TOF相结合可以得到更好的结果。当高效液相色谱和液相色谱用于多肽和蛋白质,并使用计算工具对多肽和蛋白质进行测序时,元蛋白质组学方法变得更加先进。因此,元蛋白质组学的研究是有必要的。许多科学家已经对这些方法进行了研究,但缺乏更好的质量和令人满意的结果。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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