HUBUNGAN KEKERABATAN Shorea gysbertsiana DENGAN TIGA JENIS SHOREA PENGHASIL TENGKAWANG LAINNYA BERDASARKAN PENANDA RAPD

JURNAL PEMULIAAN TANAMAN HUTAN Pub Date : 2018-12-15 DOI:10.20886/JPTH.2018.12.2.85-94

P. Sulistyawati, A. Y. Widyatmoko

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引用次数: 3

Abstract

The Shorea tengkawang species; Shorea pinanga, S. stenoptera, S. macrophylla and S. gysbertsiana known for their high-value wood and non-wood products. Shorea tengkawang produces tengkawang oil which usually used as a raw material and supporting material for the manufacture of food, pharmaceuticals and cosmetics. This study determines the genetic relationship of S. gysbertsiana to another three Shorea species uses RAPD (Random Amplified Polymorphism DNA) specific loci. There were 13 specific alleles obtained from 6 RAPD primers. Among these specific alleles, there were 8 loci shared between S. gysbertsiana and S. macrophylla, and 2 loci shared with S. stenoptera. There were no specific loci shared between S. gysbertsiana and S. pinanga. This study found one specific locus for S. gysbertsiana and one specific locus between S. stenoptera and S. pinanga. These results revealed a very close genetik relationship of S. gysbertsiana to S. macrophylla and S. stenoptera.The specific loci found in this study can be used to support the morphological identification, also for supporting conservation program of these four Shorea species.

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Shorea tengkawang种;以其高价值的木材和非木材产品而闻名的沙蚕(Shorea pinanga)、窄翅沙蚕(s.s enoptera)、大叶沙蚕(s.a macrophylla)和沙蚕(sysbertsiana)。Shorea tengkawang生产的tengkawang油通常用作食品，药品和化妆品制造的原料和配套材料。本研究利用RAPD (Random Amplified Polymorphism DNA)特异性位点确定了S. gysbertsiana与另外3种Shorea属植物的亲缘关系。6条RAPD引物共获得13个特异等位基因。在这些特异等位基因中，大叶蚜与吉斯柏氏蚜共有8个位点，窄翅蚜共有2个位点。gysbertsiana和S. pinanga之间没有特定的位点共享。本研究发现了一个特异位点，在窄翅蛾和扁翅蛾之间也有一个特异位点。这些结果表明，大叶蛾与窄翅蛾的亲缘关系非常密切。本研究所发现的特异性位点可用于形态学鉴定，也可用于支持这四种Shorea的保护计划。

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JURNAL PEMULIAAN TANAMAN HUTAN

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