SNP in intron 5 of 3-hydroxy-3-methylglutaryl coenzyme A reductase ( HMGR ) gene and its genetic effects on important economic traits in geese

Abstract

The intron 5 of the 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGR) gene was cloned and its SNP was tested by PCR-SSCP in Wanxi White goose(WW)(Anser anser). The carcass trait, meat quality trait and feather-down trait of 116 geese from WW male × Sichuan White goose(A. anser) female were mensurated and analyzed by association with SNP. The results showed that the sequence length of intron 5 of HMGR gene in goose was 704 bp, and the homology between goose and chicken was 44.0%. A SNP site was tested in the intron 5 by PCR-SSCP, and frequencies of allele A and B at the SNP site were 0.7716 and 0.2284, respectively. The effects of genotype AA on increasing chest muscle weight, gland stomach weight (P 0.05), fiber diameter of chest muscle and 1000-downs weight (P 0.01) were significant, and also significant on decreasing down hold-water percentage and chest muscle 45pH (P 0.05). The effect of genotype AB was contrary comparing with genotype AA.