Maskless direct cell patterning by laser writing

J.Y. Cheng, Hsueh-Yi Lee, M. Yen, T. Young
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引用次数: 1

Abstract

A simple and effective method for patterning cell on glass substrate is reported. A passivation layer that is capable of preventing cell adhesion was first coated onto glass surface. The passivation coatings that were tested include 3-[Bis(2-hydroxyethyl)amino]propyl-triethoxysilane, 3-Aminopropyltriethoxysilane, 1H, 1H, 2H, 2H-Perfluorodecyl-triethoxysilane, 3 -Glycidyloxypropyltriethoxysilane, Octade cyltrimethoxysilane, and Octadecyltrichlorosilane. A CO2 laser and a UV laser were used to remove the passivation layer and define patterns according to user design. Cell arrays and arbitrary pattern were demonstrated. The minimal feature attainable by the CO2 laser is about 100 mum. The minimal feature for reliable cell adhesion is found to be about 25 mum. Cells adhere and grow cleanly in the laser defined pattern. The pattern can be re-designed and fabricated easily and rapidly. Living cell staining by CMFDA and antibody staining for epidermal growth factor receptor (EGFR) detection were successfully performed on the surface bound cells. This method can be very useful for developing drug screening tools that utilize cell-based detection
激光书写的无掩模直接细胞图案
本文报道了一种简单有效的在玻璃基板上对电池进行图像化的方法。首先在玻璃表面涂上一层能防止细胞粘附的钝化层。测试的钝化涂层包括3-[双(2-羟乙基)氨基]丙基三乙基氧基硅烷、3-氨基丙基三乙基氧基硅烷、1H、1H、2H、2H-全氟癸基三乙基氧基硅烷、3-缩水氧基氧基三乙基氧基硅烷、八烷基三甲氧基硅烷和十八烷基三氯硅烷。使用CO2激光器和UV激光器去除钝化层并根据用户设计定义图案。证明了细胞阵列和任意模式。CO2激光器可达到的最小特性约为100毫微米。可靠的细胞粘附的最小特征约为25 μ m。细胞在激光定义的模式中粘附和生长干净。该图案可以方便、快速地重新设计和制作。对表面结合细胞进行CMFDA活细胞染色和表皮生长因子受体(EGFR)抗体染色。这种方法对于开发基于细胞检测的药物筛选工具非常有用
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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