APLIKASI CENDAWAN ENTOMOMOPATOGEN Cordyceps militaris PADA HAMA ULAT API (Setothosea asigna) DI PERKEBUNAN SATYA KISMA USAHA UNIT BATANG GADING MUARA BUNGO JAMBI

B. Purnama, A. Himawan, Samsuri Tarmadja
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Abstract

The aims of this research are to determine the best medium to propagation entomopathogenic fungi C. militaris, to determine effectivity and dosage of C. militaris to control pupae of S. asigna. This research was conducted in plantation of PT. Satya Kisma Usaha Unit Batang Gading, Tanah Tumbuh County, Muara Bungo District, Jambi. The research was conducted from August to September 2020. This study used a non-factorial method, which was arranged in a completely randomizing design (CRD) with 1 factor, namely growth media. The growth media are consisted of corn seeds (M1) and bran (M2) and repeated 5 times. For dosage of fungi used a non-factorial method, which was arranged in a CRD with 1 factor, namely bran media. The bran media dosages are P0 = control (100 ml aquadest/polybag), P1 = 10 g C. militaris + 100 ml aquadest/polybag, P2 = 20 g C. militaris + 100 ml aquadest/polybag, P3 = 30 g C. militaris + 100 ml aquadest/polybag P4 = 40 g C. militaris + 100 ml aquadest/polybag and all of them are repeated 4 times. The results showed that bran medium is the best medium for fungi propagation. C. militaris was effectively to control pupae of S. asigna. The best of fungi dosage is P4 = 40 g C. militaris + 100 ml aquadest/polybag. The highest pupae mortality (100%) is achieved at 12 days observation. Keywords: entomopathogenic fungi, Cordyceps militaris, bran, Setothosea asigna
本研究的目的是确定昆虫病原真菌蛹赤霉的最佳繁殖培养基,确定蛹赤霉防治白斑葡萄球菌蛹的效果和用量。本研究在占比市Muara Bungo区Tanah Tumbuh县Batang Gading的PT. Satya Kisma Usaha单位人工林进行。该研究于2020年8月至9月进行。本研究采用非析因法,采用完全随机设计(CRD), 1个因子,即生长介质。生长培养基为玉米种子(M1)和麸皮(M2),重复5次。真菌的添加量采用非因子法,按1因子即麸皮培养基排列在一个CRD中。麸皮培养基的剂量为:P0 =对照(100 ml水样/塑料袋),P1 = 10 g水样+ 100 ml水样/塑料袋,P2 = 20 g水样+ 100 ml水样/塑料袋,P3 = 30 g水样+ 100 ml水样/塑料袋,P4 = 40 g水样+ 100 ml水样/塑料袋,重复4次。结果表明,麸皮培养基是真菌繁殖的最佳培养基。蛹蠹蛾具有较好的防治效果。真菌的最佳投加量为P4 = 40 g militalis + 100 ml aquadest/塑料袋。观察12天后蛹死亡率最高(100%)。关键词:昆虫病原真菌;蛹虫草;麸皮
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