Electrochemical Characteristics of Graphene Oxide Coated on Anodized Titanium for Bone Protein Detection

Abstract

The performance of three-electrode sensor is depended on the fabrication technique of electrodes. In order to achieve the high electrical conductivity of working electrode, the methods of graphene oxide (GO) coating on anodized titanium (ATi) substrate (ATiGO) including electrodeposition, air-dried, and spin coating were studied. The cyclic voltammogram (CV) results indicated that the electrodeposition of ATiGO electrodes gave the clearly better oxidation than other coating methods and also commercial glassy carbon and platinum electrodes at a scan rate of 100 mV/s in K3Fe(CN)6 and Na2HPO4/NaH2PO4 electrolyte. The electrodeposition of ATiGO with various applied voltages (5, 10, 20 V) and durations (5, 10, 30 min) were investigated. The CV results showed that the ATiGO at 20 V and 10 min had higher peak current of oxidation than other coating conditions (not including 10 V at 30 min), but it gave the highest peak current of reduction. This study reported the preliminary data of fabrication of working electrode which would be further fabricated as a three-electrode sensor used as an implantable bone-protein detecting sensor. This sensor can be an alternative choice for early diagnosing of bone ingrowth next to an orthopedic implant.