Novel transfer of low viscosity ultraviolet light curable cyan methacrylate on saline immersed in-vitro sheep heart model and paintbrush technique

Abstract

Aim: To develop a novel technique for the transfer of cyan methacrylate on the inner and outer surfaces of the heart. Methods: Low viscosity (40cps) ultraviolet curable cyan methacrylate was mixed with commercially available dye to demonstrate the ability of transfer on the outer and inner surfaces of the in-vitro heart model. 0.5 ml of cyan methacrylate was mixed with 0.2 ml of ink, and the material was injected over the surface of the heart in dry air, and it was allowed to fix for 2-3 seconds. Subsequently, the whole preparation was immersed in saline, and it was vigorously shaken to remove unbound compound. The similar experiment was performed without cyan methacrylate, and cyanmethacrylate with high viscosity (3000 cps). By visual assessment, a significant quantity of the compound attaches to the surfaces of the heart compared to the ink alone, which was visualized after washing. After that, various techniques were investigated for efficient transfer of the compound to the inner and the outer surfaces of the heart after the tissue was soaked in saline. Of the various techniques paintbrush technique for the targeted transfer of the compound was effective. With this technique, it was very effective to transfer the compound on the epicardial and endocardial surfaces. Also, targeted areas like left atrial appendage, left ventricular inner surfaces at the origin of papillary muscle and left ventricular apex were some of the areas investigated successfully. The ultraviolet light source was a pen shaped device based on light emission diode. It was observed after the ultraviolet treatment to certain extent precipitation of the compound was observed. The experiment was repeated in 3 different samples to observe the results. Conclusion There is potential for transfer of low viscosity, ultraviolet curable cyan methacrylate for the study on the inner and outer surfaces of the heart. This could be potentially of use for transfer of growth factors or protein molecules on the surface of the heart.