Lensless microscopy through multiple determined masks

Abstract

In this paper, a transmission mode optical technique is required to achieve a super-resolved image. Basically, this technique is used for high resolution and for large field of view (FOV). The object is illuminated with deterministic mask patterns, and the object encoded information is transmitted to the sensor that is positioned at an optimum distance and records the images. A Spatial light modulator (SLM) is used as a mask and phase distribution of the SLM is changed ten times to captures ten different images. By using the angular spectrum process the captured images are backpropagated sequentially and reconstruct the super-resolved image. The use of multiple deterministic masks and Spatial Light Modulator (SLM) improved the quality of the reconstruction method. Such a technique aims to design an optical lens-less setup, working in transmission mode with portable and compact properties and to achieve a high resolution. This is much preferable to a conventional microscope in terms of resolution, the field of view, and low cost. To minimize the time of execution of the experiment, the coherent light source has been used tested. The super-resolution phase retrieval backward propagation algorithm is used to reconstruct the amplitude and phase image of the object from the captured intensity images. Advances in Microscopic Imaging III, edited by Emmanuel Beaurepaire, Adela Ben-Yakar, YongKeun Park, Proc. of SPIE-OSA Vol. 11922, 119221N · © 2021 OSA-SPIE CCC code: 1605-7422/21/$21 · doi: 10.1117/12.2615835 Proc. of SPIE-OSA Vol. 11922 119221N-1