Oxidative stress and endogenous antioxidants in normolipidemic Acute Myocardial Infarction patients

Abstract

Background: Although studies have demonstrated the role of endogenous antioxidants in the protection from the deleterious effects of oxygen free radicals in ischemia and reperfusion, there are controversial data on the correlation between endogenous antioxidants and ischemia process. Aim: The present study was planned to evaluate endogenous antioxidants in normolipidemic acute myocardial infarct (AMI) patients. Setting & Design: The serum lipid profile, albumin, uric acid, total bilirubin, malondialdehyde and conjugated dienes were determined in 165 normolipidemic patients diagnosed of AMI and 165 age-sexes matched healthy volunteers served as control. Material & Methods: Serum albumin was measured by Bromo cresol green (BCG) dye binding method, uric acid was measured by phosphotungstic acid reduction method and serum total bilirubin by Jendrassik and Grof method, serum MDA was measure by MDA method estimating TBARS and conjugated dienes by Recknagel and Glende method (with little modifications) in AMI patients and controls. Also lipid profile was analyzed enzymatically in these subjects. Statistics: The values were expressed as means ± standard deviation (SD) and data from patients and control was compared using student's't'-test. Results And Conclusion: The endogenous antioxidants were significantly decreased (p<0.001) in AMI patients compared to controls. Serum malondialdehyde and conjugated dienes were significantly (p<0.001) increased in AMI patients compared to controls. Also total cholesterol, TC: HDL-C ratio, triglycerides, LDL-cholesterol, LDL-C: HDL-C ratio and TG: HDL-C ratio were higher in AMI subjects (p<0.001) and HDL-cholesterol were lower in AMI subjects (p<0.001).