Animal Model of Non-Alcoholic Steatohepatitis in Histology Department Sohag University

Abstract

Non-alcoholic fatty liver disease (NAFLD) is characterized by the deposition of triglycerides in the hepatocytes causing their injury resulting in non-alcoholic steatohepatitis (NASH), fibrosis, and cirrhosis, as a healing response, and may progress to hepatocellular carcinoma (HCC). We aimed to establish an animal model for NAFLD/NASH by the use of a high-fat diet. A total number of 30 adult male mice were categorized into two groups; control, high-fat diet (HFD) 15 animals each. The livers of these animals were excised and processed for histological examination for the progress of NAFLD/NASH changes. At the end of the experiment, the mice were slaughtered at 11, 13, 15, and 17-week intervals. Liver specimens and blood samples from all animal groups were collected. The liver specimens were processed and stained with hematoxylin & eosin. Blood samples were processed for liver functions. Our results demonstrate that NASH was established 17 weeks after high-fat diet administration.