Upstream and downstream processing, stability and characterization studies of LigANI fragment: process development of a vaccine candidate

Abstract

Introduction: Leptospirosis is one of the most common zoonosis in the world according to WHO and the number of leptospirosis cases has been increasing over the years. Our group has been working with Leptospiral immunoglobulin-like proteins as diagnostic and subunit vaccine candidate. Initially, we cloned, expressed and purified different fragments of Lig proteins and in 2007, it was demonstrated the potential of the non-identical LigA region (LigANI) as a vaccine candidate, as it was able to protect 67-100% of the animals against lethal challenge, other groups also demonstrated the potential of this fragment as a vaccine candidate.