HYPERIMMUNIZATION OF CHICKENS EGG BREEDS FOR OBTAINING YOLK IMMUNOGLOBULINS SPECIFIC TO ESCHERICHIA COLI

Abstract

This article presents the results of studies on the selection of adjuvants and the optimal scheme for hyperimmunization of Escherichia coli laying hens to obtain specific yolk immunoglobulins. It was found that to obtain specific yolk immunoglobulins, two immunizations of chickens with formalized Escherichia coli antigen (3x109 CFU/ml) with Montanide ISA 70 or Montanide ISA 206 adjuvant are sufficient. The use of Montanide ISA 70 in an adjuvant:antigen ratio of 70:30 by weight, respectively, and Montanide ISA 206 in an adjuvant:antigen ratio of 50:50 by weight, respec-tively, is equivalent.