THE PROTECTIVE EFFECT OF N-ACETYLCYSTEINE (NAC) AGAINST OXIDATIVE STRESS ENZYME AND LIPID PROFILE FOR TOXICITY MALE RATS BY DIMETHYLNITROSAMINE

Abstract

The present study is conduct to evaluated the deleterious effects of administration of dimethylnitrosamine (DMN) on the some oxidative stress and lipid profile parameters of laboratory male rats ( Rattus rattus ), we used eighteen adult male rats randomly divided into three equal groups ( six in each). Group 1 (control) the animals treated by given oral normal saline (0.2 ml), group 2 was treated by oral dimethylnitrosamine 30 mg/kg/day, group 3 the animals were treated by oral N-acetylcysteine 300 mg/kg/day followed by dimethylnitrosamine 30 mg/kg/day. At the end of experimental period, rats were sacrificed. Blood was collected by cardiac puncture to investigate lipid profile and oxidative parameters including serum MDA, glutathione, catalase, and SOD. Results showed a significant reduction in SOD, Catalase, glutathione, and HDL, and a significant increase in MDA, LDL, total cholesterol, triglyceride, after DMN treatment, while these changes were returned to nearly normal level when they combined NAC with DMN treatment for the 30 days treatment when compared with the control group.