Jorge Díaz Heredia , Susana Alonso Güemes , Adrián Cuéllar Ayestarán , Miguel Angel Ruiz Iban
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引用次数: 1
Abstract
Objective
The addition of mesenchymal stem cells may increase the chance of healing of meniscal lesions in the avascular zone. The objective of this study is to find out if the postoperative application of the stromal vascular fraction (SVF) from adipose tissue can have an effect on vascularization and cell proliferation in the sutured meniscus.
Methods
Four pigs (8 knees) underwent a longitudinal injury of 10 mm in the avascular area of the medial meniscus in both knees. Two months later the injury was repaired on both knees with a simple suture. At the same time 50 g of abdominal fat was removed and 2 cm3 of SVF was purified (in which it was found to contain approximately 1-5 × 106 mesenchymal stem cells). This was injected into one of the knees of each pig 4 h after the surgery (SVF group), using the other knee as the control group. The animals were sacrificed after 15 days. The following histological parameters with hematoxylin-eosin staining were evaluated: degree of healing, neo-vascularization of tissue and the proliferation of fibro-chondrocytes around the lesion and the presence of new chondrocytes.
Results
The healing rate (unhealed / partial healing / healed) for the SVF group was 1/1/2, and was 2-2-0 for control group (non-significant differences). The degree of neo-vascularization was higher in the SVF group (3.25 vs 2). The degree of fibro-chondrocytes proliferation was greater in the SVF group (2.5 vs 1.75). The formation of isogenic chondrocytes was observed in 2 menisci of the SVF group and none in the control group.
Conclusions
The postoperative intra-articular injection of SVF might increase the neovascularization, the proliferation of fibro-chondrocytes and the repopulation of chondrocytes 15 days after the repair of meniscal injury in pigs.
Evidence level
Experimental study.
Clinical relevance
A system for the use of stem cells is presented that might be easier to implement in humans that cell cultures.
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