Antibiotic sensitivity of Mycoplasma bovis and other respiratory pathogens isolated from pneumonic lung samples in a calf rearing unit in Turkey

Animal Husbandry, Dairy and Veterinary Science Pub Date : 1900-01-01 DOI:10.15761/ahdvs.1000150

U. Ozdemir, Turkyilmaz Ma, N. Raj

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引用次数: 1

Abstract

Mycoplasma bovis, a serious cause of bovine respiratory disease often associated with other bacteria and viruses, is prevalent worldwide including Turkey. It is difficult to control because antibiotics are becoming increasingly ineffective and commercial vaccines are not available. The aims of this study were to detect infective agents causing respiratory disease in a calf rearing unit in Turkey to determine antibiotic susceptibilities of the strains isolated and to carry out molecular typing. In diseased lungs from calves dying of pneumonia we found M. bovis to be present in all samples of acute and chronic diseased tissue. Mannheimia haemolytica was detected mostly in chronic tissue (39 %) while the reverse was true for Pasteurella multocida with most isolations from acute tissue (25 %). High minimum inhibitory concentrations (MIC) were seen for most antibiotics against M. bovis: tylosin, tilmicosin, erythromycin, chloramphenicol, oxytetracycline and ciprofloxacin with MIC50s of >32 μg/ml, intermediate-sensitivity to florfenicol, spectinomycin, danofloxacin, enrofloxacin and marbofloxacin with MIC50 of 8 μg/ml, and susceptible to lincomycin, clindamycin and tulathromycin with MIC50 of 1, 0.25, 0.25 μg/ml respectively. While all tested isolates of both M. haemolytica and P. multocida were resistant to gentamicin, P. multocida isolates showed resistance rates of 100 % to erythromycin and tylosin, 88 % to trimethoprim-sulphametoxasol, 75% to tetracycline and tilmicosin, 50 % to tulathromycin and enrofloxacin. The resistance rates of M. haemolytica isolates were 90 % to erythromycin, 75 % to tylosin, 64 % to tetracycline, 55 % to trimethoprim-sulphametoxasol, 36 % to tilmicosin, 18 % to enrofloxacin, 9 % to marbofloxacin, florfenicol, ampicillin and penicillin. Finally, M. bovis could be divided into two distinct genetic clusters by molecular typing tests. *Correspondence to: Nıcholas RAJ, The Oaks, Nutshell Lane, Farnham Surrey, UK, E-mail: robin.a.j.nicholas@gmail.com

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从土耳其犊牛饲养单位肺样本中分离的牛支原体和其他呼吸道病原体的抗生素敏感性

牛支原体是牛呼吸道疾病的一种严重病因，通常与其他细菌和病毒有关，在包括土耳其在内的世界范围内普遍存在。它很难控制，因为抗生素正变得越来越无效，而且没有商业化的疫苗。本研究的目的是检测在土耳其犊牛饲养单位引起呼吸道疾病的感染因子，以确定分离菌株的抗生素敏感性并进行分子分型。在死于肺炎的小牛的病变肺中，我们发现牛分枝杆菌存在于所有急性和慢性病变组织样本中。溶血性曼海姆病主要在慢性组织中检测到(39%)，而多杀性巴氏杆菌则相反，大多数从急性组织中分离到(25%)。大多数抗生素对牛分枝杆菌具有较高的最低抑菌浓度(MIC):泰洛新、替米霉素、红霉素、氯霉素、土霉素和环丙沙星的MIC50值>32 μg/ml，对氟苯尼科尔、大观霉素、达诺沙星、恩诺沙星和马布沙星的MIC50值为8 μg/ml，对林可霉素、克林霉素和图拉霉素的MIC50值分别为1、0.25、0.25 μg/ml。溶血支原体和多杀假单胞菌对庆大霉素均有耐药率，多杀假单胞菌对红霉素和泰霉素的耐药率为100%，对甲氧苄啶磺胺醇的耐药率为88%，对四环素和替米霉素的耐药率为75%，对图拉霉素和恩诺沙星的耐药率为50%。溶血支原体对红霉素的耐药率为90%，对泰洛菌素的耐药率为75%，对四环素的耐药率为64%，对甲氧苄氨嘧啶的耐药率为55%，对替米霉素的耐药率为36%，对恩诺沙星的耐药率为18%，对马布沙星、氟苯尼可、氨苄西林和青霉素的耐药率为9%。最后，通过分子分型测试，牛分枝杆菌可分为两个不同的遗传群。*通信:Nıcholas RAJ, The Oaks，果壳巷，萨里郡，英国，E-mail: robin.a.j.nicholas@gmail.com

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Animal Husbandry, Dairy and Veterinary Science

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