Deciphering the structural community from the Deulajhari hot spring using the next-generation sequencing

Abstract

Deciphering the microbial diversity of the Deulajhari thermal springs is the major goal of our study. In our study the taxonomic description of the bacterial community structure was deduced from the 1.52 Gb metagenomics sequence size from the Deulajhari hot spring located in the Angul district of Odisha. Covered with the dense foliage, it has a high temperature, i.e. 69°C and alkaline pH i.e. 8.09. Various physiochemical parameter analysis of the sediment like; electro conductivity (0.025dSm-1), total organic carbon content (0.356%), nitrogen (125 kg/ha), phosphorus (7.88 kg/ha) and potassium (169.34 kg/ha) were done. Sediment sample of the Deulajhari hot spring was further processed for the 16S rRNA V3-V4 region by the amplicon metagenome sequencing from community DNA. Approximately 88.12% of the total microbial diversity was represented by the Proteobacteria followed by Bacteroidetes 10.76%, Firmicutes 0.35%, Spirochetes 0.18%, Thermi 0.13% and chloroflexi 0.11% at the phylum level. Thus through the metagenomics sequencing of the Deulajhari hot spring using IIllumina platform, we represent the complete microbial community structure present there in respective of their allocation, profusion and diverse coexisting microbiota.