G: Principles and Methods

Sixth Edition, Richard A. Johnson, Gouri K. Bhattacharyya, John Wiley, Ellen Keohane, Anne Scanlan-Rohrer
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Abstract

It has been suggested that naturally occurring copper and zinc concentrations in deep seawater are toxic to marine organisms when the free ion forms are overabundant. The effects of micronutrients on the growth of gametophytes of the ecologically and commercially significant giant kelp Macrocystis pyrifera were studied in defined media. The results indicate that toxic copper and zinc ion concentrations as well as cobalt and manganese deficiencies may be among the factors controlling the growth of marine organisms in nature. A least squares fit of gametophytic growth data in the defined medium generated the expression (1) where Y is mean gametophytic length in micrometers. The fit of the experimental data to Eq. (1) was considered excellent. Here, several variables are important for predicting growth. 18xCo 6xCux 2 Zn 6xCux 2 Mn 7xZnxCu 15x 2 Zn 27x 2 Mn 12x 2 Cu Y 136 8xMn 5xCu 7xCo Source: J. S. Kuwabara, “Micronutrients and Kelp Cultures: Evidence for Cobalt and Manganese Deficiency in Southern California Deep Sea Waters,” Science, 216 (June 11, 1982), pp. 1219–1221. Copyright © 1982 by AAAS. © David Hall/Photo Researchers, Inc. c12.qxd 10/15/09 11:18 AM Page 486
G:原理和方法
有人认为,当深层海水中自然存在的铜和锌的自由离子形式过多时,对海洋生物是有毒的。在确定的培养基中,研究了微量营养素对具有生态和商业意义的巨藻(Macrocystis pyrifera)配子体生长的影响。结果表明,有毒的铜和锌离子浓度以及钴和锰的缺乏可能是控制自然界海洋生物生长的因素之一。对所定义的培养基中的配子体生长数据进行最小二乘拟合,得到式(1),其中Y为配子体平均长度,单位为微米。实验数据与Eq.(1)的拟合性极佳。在这里,有几个变量对预测增长很重要。来源:J. S. Kuwabara,“微量营养素和海藻培养:南加州深海水域钴和锰缺乏的证据”,《科学》,216(1982年6月11日),第1219-1221页。美国科学促进会版权所有©1982。©David Hall/Photo Researchers, Inc. c12。qxd 10/15/09 11:18 AM 486页
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