Dynamic washout multiple droplet lab on chip routing: Dynamic washing technique

Abstract

Digital microfluidic biochip (DMFB) is a technology that has just came up with the aim to attenuate droplets activity on a chip. By manipulating droplets with negligible volumes, the DMFB provides susceptibility with comparatively less human errors than the former routing methods. When two droplets are sharing a same routing path, the second droplet may be contaminated due to the leftovers of the first droplet which affects the assay. To avoid cross contamination 1. A DMFB need to be periodically washed out. 2. Contamination aware routing path need to be calculated. This work attends to utilize both the modified version of the solution. This project work attempts to propose a simultaneous wash out routing algorithm for dynamic parallel droplet testing with reduced timing overhead. A reconfigurable DMFB (Digital Microfluidic Biochip) test bed architecture with N × N pin structure will be designed using HDL. Parallel droplets will be introduced using different input pin channels with addition to that a wash out droplet will also be introduced and kept idle initially. When the destination pins for the actual droplets is configured, shortest path routing prediction will take place in parallel with a multi-objective modified swarm intelligence algorithm which is initiated to predict the routing path of the wash droplet to avoid cross contamination possibilities for the testing droplets. The second routing algorithm is dedicated for wash droplet through which is analyzes the first (droplet) routing result to find possible cross contamination. The modified swarm routing will be gated in case of non-contaminated routing path is already predicted in the first routing which saves the circuit power dissipation.