The Anti-Hypoxia Protective Effect of WYY026B by Interacting with Hemoglobin in Red Blood Cells

Abstract

Background Exposure to hypoxia may experience damage to the heart, brain, lungs and other organs due to the low inspired O2. The improvement of endurance of human in resisting hypoxia and reducing hypoxia‑induced organ damage is an urgent problem to be solved. WYY026B is a honokiol derivatives by structural modification, and exhibits very potent pharma‑ cological activities and medicinal properties in brain ischemia/reperfusion (I/R) injury. Methods We used hypoxia model cerebral I/R model to identify the anti-hypoxia effect of WYY026B. High performance liquid chromatograph and immunofluorescence assay were used to detect the relationship between WYY026B and Red blood cells (RBCs). Interaction between WYY026B and hemoglobin (Hb) were validated by surface plasmon resonance assay (SPR). Results We demonstrated that WYY026B increased arterial oxygen saturation (SaO2) and arterial partial pressure of oxygen un‑ der hypoxia stress to improve the hypoxia tolerance. Blood gas analysis showed that WYY026B significantly decreased cervical venous SaO2 and increased arteriovenous oxygen partial pressure difference in I/R model. Nissl´s staining in‑ dicated that WYY026B significantly reduced the degree of hypoxia in brain tissue. A series of tests in vitro revealed that WYY026B could combine with RBCs. SPR assays showed that WYY026B interacted with Hb. Conclusion Overall, WYY026B maybe a new drug to enhance the ability of Hb to carry oxygen and to protect brain from the hypoxia injury