Fast Repetition Rate (FRR) Fluorometer For Making In Situ Measurements Of Primary Productivity

Zbigniew, Kolber, Paul G. Falkowski
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引用次数: 33

Abstract

Understanding the ocean carbon cycle and predicting how climate-induced changes in ocean circulation will affect ocean productivity requires that (a) primary productivity be measured with high spatial and temporal resolution, and (b) natural variability in primary productivity be parameterized with regardto environmental factors such as nutrient availabuity, irradiance, and temperature. Instrumentation to measure primary productivity from the stimulated in vivo fluoresence of phytoplankton chlorophyll is currendy being developed at Brookhaven National Laboratory. The instrumentation is based on fast repetition rate (FRR) fluorometry, and provides a robust technique for deriving the photosynthetic rates in situ. Moreover, the FRR methodology directly measures several photosynthetic parameters such as effective absorption cross- section, photo-conversion efficiency, and turnover time of photosynthesis, and relate them to primary productivity. Since photosynthetic parameters are affected by environmental factors such as fight and nutrient availability, the relationship between these parameters and primary productivity can be established. By understanding such relationships, prognostic models of primary productivity can be developed and parameterized.
用于原位测量初级生产力的快速重复率荧光计
了解海洋碳循环并预测气候引起的海洋环流变化将如何影响海洋生产力,需要(a)以高时空分辨率测量初级生产力,以及(b)将初级生产力的自然变率与诸如养分有效性、辐照度和温度等环境因素参数化。布鲁克海文国家实验室目前正在开发测量浮游植物叶绿素体内荧光激发的初级生产力的仪器。该仪器是基于快速重复率(FRR)荧光测定法,并提供了一个强大的技术,以获得原位光合速率。此外,FRR方法直接测量光合作用的有效吸收截面、光转换效率和周转时间等几个光合参数,并将它们与初级生产力联系起来。由于光合参数受战斗和养分有效性等环境因素的影响,因此可以建立光合参数与初级生产力之间的关系。通过了解这种关系,可以开发初级生产力的预测模型并将其参数化。
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