A Silicon-Based Single-Cell Electroporation Microchip for Gene Transfer

Younghak Cho, B. Pioufle, N. Takama, Beomjoon Kim
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引用次数: 2

Abstract

In our contribution, we present the fabrication of electroporation microchip in detail. The practical experiments of single-cell electroporation with our fabricated microchip will be carried out. Electroporation test efficiency and cell viability tests will be provided. This device enables to reduce the size of samples and thus the use of small amount of reagents. It may also permit to avoid cell separation (transfected cells versus non transfected cells) encountered when traditional bulk electroporation is held
用于基因转移的硅基单细胞电穿孔微芯片
在本文中,我们详细介绍了电穿孔微芯片的制备。用自制的微芯片进行单细胞电穿孔的实际实验。将提供电穿孔测试效率和细胞活力测试。该装置可以减少样品的尺寸,从而使用少量试剂。它还可以避免传统的大块电穿孔时遇到的细胞分离(转染细胞与未转染细胞)
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