The Effect of Herbal Based Potent Antioxidants on Chemotherapy Resistant Ovarian Adenocarcinoma Cell Lines

Abstract

This study investigates conventional delivery of thymoquinone (TQ) and epigallocatechin-3-gallate (EGCG) when applied to Caov-3 and SK-OV-3 ovarian like cell lines. Culturing the cells and assessment of functional activities were conducted following standard lab protocols. The results revealed: (1) an increase in the SK-OV-3 cell protein levels following treatment with TQ+EGCG which was statistically different (p<;0.05) at 24 and 48 hours; (2) nitric oxide levels were statistically different (p<;0.05) following the administration of EGCG and TQ+EGCG at 24 and 48 hours for Caov-3 and TQ at 72 hours for SK-OV-3; and (3) glutathione levels were statistically different (p<;0.05) following the administration of TQ and EGCG to the SK-OV-3 cell line at 72 hours. Overall conclusion of this study demonstrates that exposure of potent herbal based anti-oxidant extracts may potentially interfere with the functional and mitotic activity of ovarian cancer cells.