{"title":"The measurement of cholecystokinin.","authors":"J F Rehfeld","doi":"10.1136/jcp.s1-8.1.26","DOIUrl":null,"url":null,"abstract":"Measurement of hormones in biological fluids is an obvious prerequisite for evaluation of their physiological and pathophysiological significance. Of the steadily increasing list of gastrointestinal hormones, cholecystokinin (CCK) was the third to be discovered (Ivy and Oldberg, 1928). Cholecystokinin, secretin, and gastrin constitute the classical triad of gut hormones which, until recently, was assumed to exert the entire humoral control of digestion (Grossman, 1970a). While it is now possible to measure the plasma concentration of gastrin, secretin, and most of the other chemically defined hormonal peptides from the gut (reviewed by Rehfeld, 1978a), all attempts to measure CCK in plasma have, in the author's opinion, failed. An interest in the molecular nature of gastrin and gastrin-like peptides, and experience of assays for a number of gastrointestinal and other hormones, led me to establish the radioimmunoassay of CCK. After initial difficulties with the labelling of CCK had been overcome, sensitive and sequence-specific radioimmunoassays for CCK were developed (Rehfeld, 1978b). However, for several reasons, these assays proved unsatisfactory when applied to plasma. As foreseen several years ago using crossreacting gastrin assays (unpublished data), CCK occurs in several forms and their concentration in plasma is very low. Moreover, plasma proteins interfere greatly in the assay, and some plasma enzymes probably degrade CCK. These problems still have to be overcome. Nevertheless, it is hoped that the following account may be of some value.","PeriodicalId":75995,"journal":{"name":"Journal of clinical pathology. Supplement (Association of Clinical Pathologists)","volume":"8 ","pages":"26-30"},"PeriodicalIF":0.0000,"publicationDate":"1978-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1136/jcp.s1-8.1.26","citationCount":"4","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of clinical pathology. Supplement (Association of Clinical Pathologists)","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1136/jcp.s1-8.1.26","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 4
Abstract
Measurement of hormones in biological fluids is an obvious prerequisite for evaluation of their physiological and pathophysiological significance. Of the steadily increasing list of gastrointestinal hormones, cholecystokinin (CCK) was the third to be discovered (Ivy and Oldberg, 1928). Cholecystokinin, secretin, and gastrin constitute the classical triad of gut hormones which, until recently, was assumed to exert the entire humoral control of digestion (Grossman, 1970a). While it is now possible to measure the plasma concentration of gastrin, secretin, and most of the other chemically defined hormonal peptides from the gut (reviewed by Rehfeld, 1978a), all attempts to measure CCK in plasma have, in the author's opinion, failed. An interest in the molecular nature of gastrin and gastrin-like peptides, and experience of assays for a number of gastrointestinal and other hormones, led me to establish the radioimmunoassay of CCK. After initial difficulties with the labelling of CCK had been overcome, sensitive and sequence-specific radioimmunoassays for CCK were developed (Rehfeld, 1978b). However, for several reasons, these assays proved unsatisfactory when applied to plasma. As foreseen several years ago using crossreacting gastrin assays (unpublished data), CCK occurs in several forms and their concentration in plasma is very low. Moreover, plasma proteins interfere greatly in the assay, and some plasma enzymes probably degrade CCK. These problems still have to be overcome. Nevertheless, it is hoped that the following account may be of some value.