Contrary microscope: opportunities and advantages: optical design

Abstract

In the classical layout of the light microscope, when the objective is used as a projection system, and eyepiece as an observation, the redistribution of overall and functional parameters is proposed. In the classical layout typically, own linear magnification for objectives is from 2.5 to 100x, and eyepieces from 5 to 30x. It is proposed to use objectives of magnification of not more than 10-15x but having extremely achievable input numerical apertures. In this case, the range of increases of the eyepieces expands to 50x and even 100x. We offer examples of optical designs of objectives and eyepieces. The objective 10x magnification has NA=0.90 and 12.5x NA=1.20 of the water immersion. The linear field in the space of images is 20 mm. In addition, examples of optical designs of eyepieces 50x and 100x are offered too.