Combined Effect of Arbuscular Mycorrhiza, Rhizobium and Sclerotium rolfsii on Grass Pea ( Lathyrus sativus )

The Agriculturists Pub Date : 2017-08-04 DOI:10.3329/AGRIC.V15I1.33438

M. A. Rahman, Me Ali, F. Alam, M. Islam, M. Bhuiyan

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引用次数: 3

Abstract

The present study was carried out to evaluate the combined effect of indigenous arbuscular mycorrhizal (AM) fungi, Rhizobium and Sclerotium rolfsii on Grasspea ( Lathyrus sativus ) in the net house of Soil Science Division, Bangladesh Agricultural Research Institute, Joydebpur, Gazipur during 2014-2015 through 2015-2016. The experiment was designed in RCBD with 8 treatments and 4 replications. Grasspea variety BARI Khesari-1 was used as a test crop. Peat based rhizobial inoculum (BARI RLs-10) was used in this experiment @ 50 g kg -1 seed. The AM fungi used in this experiment were Glomus fusianum, Glomus macrocarpum, Glomus warcuppi, Acaulospora foveata, Acaulospora denticulate, Gigaspora albida, Gigaspora rosea, Glomus spp. etc. Soil based AM inoculum containing about approximate 252 spores and infected root pieces of the host plant was used pot -1 . There were eight treatments viz. T 1 : Arbuscular mycorrhiza (AM), T 2 : Rhizobium , T 3 : AM + Rhizobium , T 4 : Sclerotium rolfsii , T 5 : Sclerotium rolfsii + AM, T 6 : Sclerotium rolfsii + Rhizobium , T 7 : Sclerotium rolfsii + AM + Rhizobium and T 8 : Control. Dual inoculation (AM + Rhizobium ) significantly increased germination (%), nodule number and dry weight, root colonization by AM fungi and spore population in rhizosphere soils of grasspea compared to single inoculation or any other treatments. Dual inoculation increased germination after 23 DAS (20% in 2014-2015 and 23% in 2015-2016) compared to control. It increased nodule number plant -1 (172% in 2014-2015 and 72% in 2015-2016) over AM treatment, and (112% in 2014-2015 and 26% in 2015-2016) over Rhizobium treatment. It also increased root infection (20% in 2014-2015 and 56% in 2015-2016) over AM treatment, and (200% in 2014-2015 and 100% in 2015-2016) over Rhizobium treatment. It reduced foot and root rot disease (48% in 2014-2015 and 44% in 2015-2016) compared to control. On the contrary, Sclerotium rolfsii + Rhizobium, Sclerotium rolfsii + AM, and Sclerotium rolfsii + AM + Rhizobium reduced 12-17%, 16-20% and 28-31% foot and root rot disease, respectively compared to only Sclerotium rolfsii treatment. Therefore, use of these bio-control agents could be promoted as an active component of bio-intensive Integrated Disease Management Program (IDMP) under organic mode. The Agriculturists 2017; 15(1) 143-155

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丛枝菌根、根瘤菌和菌核菌对草豆的联合防治作用

本研究在2014-2015年至2015-2016年期间，在加齐浦尔Joydebpur孟加拉国农业研究所土壤科学处的网房中，评估了土生丛枝菌根(AM)真菌、根瘤菌和罗尔夫菌核菌对草地(Lathyrus sativus)的联合效应。试验采用RCBD设计，8个处理，4个重复。以牧草品种BARI Khesari-1为试验作物。本试验采用泥炭根瘤菌接种剂(BARI RLs-10)，接种量为50 g kg -1粒种子。本实验使用的AM真菌有Glomus fususianum、Glomus macrocarpum、Glomus warcuppi、Acaulospora foveata、Acaulospora dendenulate、Gigaspora albida、Gigaspora rosea、Glomus spp等。盆栽-1采用含约252个孢子和侵染的寄主根片的土基AM接种物。共有8个处理，即t1:丛枝菌根(AM)、t2:根瘤菌、t3: AM +根瘤菌、t4:罗尔夫菌核菌、t5:罗尔夫菌核菌+ AM、t6:罗尔夫菌核菌+根瘤菌、t7:罗尔夫菌核菌+ AM +根瘤菌和t8:对照。与单次接种或其他处理相比，双接种(AM +根瘤菌)显著提高了禾草根际土壤中AM真菌的发芽率、根瘤数和干重、根定植和孢子数量。与对照相比，双重接种在23 DAS后使种子萌发率提高(2014-2015年为20%，2015-2016年为23%)。比AM处理增加了1株(2014-2015年为172%，2015-2016年为72%)，比根瘤菌处理增加了112%，比根瘤菌处理增加了26%。它也比AM处理增加了根感染(2014-2015年为20%，2015-2016年为56%)，比根瘤菌处理增加了(2014-2015年为200%，2015-2016年为100%)。与对照相比，它减少了足部和根腐病(2014-2015年为48%，2015-2016年为44%)。与单纯菌核处理相比，菌核+根瘤菌、菌核+ AM和菌核+ AM +根瘤菌分别减少了12-17%、16-20%和28-31%的足腐病和根腐病。因此，在有机模式下，这些生物防治剂可以作为生物密集型综合疾病管理计划(IDMP)的有效组成部分加以推广。农学家2017;15 (1) 143 - 155

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The Agriculturists

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