AMELIORATIVE EFFECT OF BETA CAROTENE AGAINST TITANIUM DIOXIDE NANOPARTICLES REPRODUCTIVE TOXICITY ON TESTIS AND EPIDIDYMIS OF MALE ALBINO MICE MUS MUSCULUS

Abstract

The present study aimed to evaluate the improving or ameliorative effect of beta carotene on titanium dioxide nanoparticles induced testicular toxicity at the histological level. Forty adult healthy male albino mice weighting between 30-37gm and aged 12 to 15. Animals were randomly grouped in to four groups with ten mice in each: the first one was administered normal saline, whereas second group mice were administered 10 mg\kg body weight of beta carotene, third group were given 300 mg\kg body weight of titanium dioxide solution, last fourth group were administered 300 mg\kg body weight of titanium dioxide solution and 10 mg\kg body weight of beta carotene after two hours of every titanium uptake after ten days of pre protective administration of10 mg\kg body weight beta carotene ; all for thirty five days. Results exhibited that the histological toxic effects induced by A 300 mg\kg titanium dioxide nanoparticles administered mice testis showing germinal epithelium sloughing and odema with irregular spermatogenesis .and showing That epididymal epithelium exhibiting lipid vacuoles in the supranuclear region of the principal cells, with papillary infoldings lined by columnar cells and occur hyperplasia of clear cells, Some lumens were devoid of sperm with few cellular debris, also showed atrophy in the smooth muscle between ducts with Inflammatory infiltrate in connective tissue. A group treated with 300 mg\kg titanium dioxide NPs plus 10 mg\kg body weight administered mice that testis and epididymis showing an intact of histologic architecture. And the above effects were ameliorated by the administration of beta carotene, and the pre protective effect of beta carotene increased that effect. In conclusion Beta carotene has improving effects against the histological damage in testis and epididymis and the arrest in spermatogenesis that resulted from the toxicity of titanium dioxide nanoparticles as it repair the damaged parameters and obtaining control like features